Kallio T J, Vauhkonen M V, Peltonen J I, Karaharju E O
Department of Orthopedics and Traumatology, Helsinki University Central Hospital, Finland.
Acta Orthop Scand. 1994 Aug;65(4):467-71. doi: 10.3109/17453679408995494.
Osteotomy of the distal radius and gradual distraction were performed on 20 sheep. Bone matrix synthesized between the osteotomized bone ends was biochemically characterized at 3, 5, 7, and 14 days after the start of distraction. The deposition of mineral was negligible during the 14-day period. No change was observed in the amount of organic matrix, which comprised 15 +/- 1.4 percent of the wet weight of the tissue. The amount of total protein of the wet weight in the matrix increased from 4.6 to 7.6 percent between 5 and 7 days of distraction. During the first 7 days, the nonprotein component decreased from 11 to 7.3 percent. The proportion of collagen of the total matrix protein increased gradually from 29 to 59 percent. In view of the patterns of the cyanogen bromide peptides of the matrix proteins, the heteropolymer type I[1(I)2(2)(I)1] collagen was consisted to be the principle fibrillar collagen synthesized in the distraction gap. The results suggest that tension-stress markedly affects the synthesis of fibrillar collagen towards the formation of mature bone matrix. In this process, a preliminary lag phase probably exists during which the capacity for protein synthesis is low while the synthesis of Type I collagen has started. In the secondary phase an augmented synthesis of Type I collagen takes place.
对20只绵羊进行了桡骨远端截骨术并逐步牵张。在牵张开始后的第3、5、7和14天，对截骨骨端之间合成的骨基质进行了生化特性分析。在这14天期间，矿物质沉积可忽略不计。有机基质的量未观察到变化，其占组织湿重的15±1.4%。基质中湿重总蛋白的量在牵张的第5至7天从4.6%增加到7.6%。在最初的7天里，非蛋白成分从11%下降到7.3%。总基质蛋白中胶原蛋白的比例从29%逐渐增加到59%。鉴于基质蛋白的溴化氰肽模式，异聚体I型[1(I)2(2)(I)1]胶原蛋白被认为是在牵张间隙中合成的主要纤维状胶原蛋白。结果表明，张力应力显著影响纤维状胶原蛋白的合成，促进成熟骨基质的形成。在这个过程中，可能存在一个初始延迟期，在此期间蛋白质合成能力较低，而I型胶原蛋白的合成已经开始。在第二阶段，I型胶原蛋白的合成增加。
