Kojima H, Shiwa M, Kamide Y, Moriyama H
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Acta Otolaryngol. 1994 Jul;114(4):423-9. doi: 10.3109/00016489409126081.
The roles of epidermal growth factor (EGF) and epidermal growth factor receptor (EGF-R) in the proliferation and progression of the epithelium of middle ear cholesteatoma were studied. An attempt was made to elucidate the site and degree of localization of the EGF mRNA and EGF-R mRNA in the epithelium of the cholesteatoma by means of the non-radioactive in situ hybridization method. Ten cholesteatoma specimens excised during operations and six normal skin specimens (control) collected from the external ear canal were used in this study. The signal of the EGF mRNA was slightly expressed in part of the basal cells in only one of the six control specimens, while the signal was strongly expressed along the basal cells of the cholesteatoma epithelium in five of the ten specimens. The signal of the EGF-R mRNA was observed along the basal cell layer in five of the six control specimens, while the signal was strongly expressed in all layers of the cholesteatoma epithelium in all ten specimens. The expression was especially marked in the basal cell layer. These findings suggest the possibilities that abnormal expression of the EGF-R mRNA in nearly entire epithelial layers of cholesteatoma is due to overexpression of EGF-R gene, and that there is a mechanism of epithelial basal cell proliferation through an autocrine regulatory system via EGF and EGF-R.
研究了表皮生长因子（EGF）和表皮生长因子受体（EGF-R）在中耳胆脂瘤上皮细胞增殖和进展中的作用。试图通过非放射性原位杂交方法阐明EGF mRNA和EGF-R mRNA在胆脂瘤上皮细胞中的定位部位和程度。本研究使用了手术切除的10例胆脂瘤标本和从外耳道收集的6例正常皮肤标本（对照）。在6例对照标本中，只有1例的部分基底细胞中EGF mRNA信号呈轻度表达，而在10例标本中的5例中，胆脂瘤上皮细胞的基底细胞中EGF mRNA信号呈强表达。在6例对照标本中的5例中，沿基底细胞层观察到EGF-R mRNA信号，而在所有10例标本中，胆脂瘤上皮细胞的所有层中EGF-R mRNA信号均呈强表达，在基底细胞层表达尤为明显。这些发现提示，胆脂瘤几乎整个上皮层中EGF-R mRNA的异常表达可能是由于EGF-R基因的过度表达，并且存在通过EGF和EGF-R的自分泌调节系统导致上皮基底细胞增殖的机制。
