Shiwa M, Kojima H, Moriyama H
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
J Laryngol Otol. 1998 Aug;112(8):750-4. doi: 10.1017/s0022215100141593.
This study aims at elucidating the role of cytokines in the mechanism of proliferation of cholesteatoma epithelium by investigating the mode of expression of epidermal growth factors, such as TGF-alpha. The subjects of this study were patients who had undergone operation for middle ear cholesteatoma. Skins of the bone region of the external ear canal (normal skin) of the same patients were used as the negative control. The mode of expression of TGF-alpha was studied by immunohistochemistry and in situ hybridization. In the immunohistochemical study, there were no conspicuous differences observed between cholesteatoma tissues and normal skin. After in situ hybridization, expression of TGF-alpha mRNA was mainly observed in the epidermal basal cell layer in the normal skin, while in the cholesteatoma epidermis with severe inflammatory cell infiltration, expression of TGF-alpha mRNA was observed up to layers superior to the basal cell layer. The expression of TGF-alpha mRNA is greatly affected by subepithelial connective tissue, strongly suggesting involvement of paracrine regulation in proliferation of cholesteatoma epithelium.
本研究旨在通过研究表皮生长因子如转化生长因子-α（TGF-α）的表达模式，阐明细胞因子在胆脂瘤上皮细胞增殖机制中的作用。本研究的对象为接受过中耳胆脂瘤手术的患者。将同一患者外耳道骨部皮肤（正常皮肤）用作阴性对照。采用免疫组织化学和原位杂交技术研究TGF-α的表达模式。在免疫组织化学研究中，胆脂瘤组织与正常皮肤之间未观察到明显差异。原位杂交后，正常皮肤中TGF-α mRNA的表达主要见于表皮基底细胞层，而在有严重炎性细胞浸润的胆脂瘤表皮中，TGF-α mRNA的表达可见于基底细胞层以上各层。TGF-α mRNA的表达受上皮下结缔组织的显著影响，强烈提示旁分泌调节参与胆脂瘤上皮细胞的增殖。
