Kojima H, Matsuhisa A, Shiwa M, Kamide Y, Nakamura M, Ohno T, Moriyama H
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Arch Otolaryngol Head Neck Surg. 1996 Feb;122(2):157-60. doi: 10.1001/archotol.1996.01890140043009.
To understand the interaction between cholesteatoma epithelium and subepithelial connective tissue as a paracrine regulation by keratinocyte growth factor.
Preparation of a specific DNA probe from human fetal fibroblast and detection of localization of keratinocyte growth factor messenger RNA in subepithelial granulation tissue of middle-ear cholesteatoma by a nonradioactive in situ hybridization method.
The cholesteatoma specimens were excised from 12 patients during surgery. Normal skin specimens collected from the external ear canal of six patients were used as controls.
Signals specific for keratinocyte growth factor messenger RNA were not expressed in the normal skin of the external ear canal, but were observed in fibroblasts of subepithelial connective tissue of cholesteatoma specimens. Signals were observed only in specimens collected from patients whose subepithelial connective tissue was thick and proliferated and whose inflammation was strong.
A paracrine regulation mechanism involving keratinocyte growth factor may exist for proliferation of epithelium of cholesteatoma. The subepithelial connective tissue of cholesteatoma may play an important role in the proliferation and development of the cholesteatoma, especially under inflammatory conditions.
了解胆脂瘤上皮与上皮下结缔组织之间的相互作用，即角质形成细胞生长因子的旁分泌调节作用。
从人胎儿成纤维细胞制备特异性DNA探针，采用非放射性原位杂交方法检测中耳胆脂瘤上皮下肉芽组织中角质形成细胞生长因子信使核糖核酸的定位。
手术中从12例患者切除胆脂瘤标本。从6例患者外耳道采集的正常皮肤标本用作对照。
角质形成细胞生长因子信使核糖核酸的特异性信号在外耳道正常皮肤中未表达，但在胆脂瘤标本的上皮下结缔组织成纤维细胞中观察到。仅在从上皮下结缔组织增厚、增殖且炎症强烈的患者采集的标本中观察到信号。
胆脂瘤上皮增殖可能存在涉及角质形成细胞生长因子的旁分泌调节机制。胆脂瘤的上皮下结缔组织可能在胆脂瘤的增殖和发展中起重要作用，尤其是在炎症条件下。
