Zhao Y X, He X Y, Jiang M, Xie J W, Xie T Q, Ren L Q
Department of Pharmacy, Lanzhou General Hospital of Lanzhou Command.
Yao Xue Xue Bao. 1994;29(7):550-2.
A high performance liquid chromatographic assay was developed for determination of the plasma concentration of hexamethylene bisacetamide (HMBA) in rabbit. Plasma samples were vortex-mixed with 0.3 ml of methanol and centrifuged at 10000 r/min for 5 min. A 20 microliters aliquot of the supernatant was injected into the HPLC system. Analysis was performed on a Du Pont Zorbax C18 column (250 mm x 4.6 mm, 10 microns) with a precolumn (50 mm x 4.6 mm, 10 microns, YWG-C18). The eluent was methanol--water (30:70), with flow rate of 1.0 ml.min-1 and UV detection at 210 nm. The limit of detection was 1.0 microgram.ml-1 in plasma and the calibration curve was linear over the range 5-1000 micrograms.ml-1 (r = 0.9988). The HPLC assay was applied for studying the pharmacokinetics of HMBA in rabbit.
建立了一种高效液相色谱法用于测定兔血浆中六亚甲基双乙酰胺（HMBA）的浓度。血浆样品与0.3 ml甲醇涡旋混合，然后以10000转/分钟的速度离心5分钟。取20微升上清液注入高效液相色谱系统。分析在Du Pont Zorbax C18柱（250 mm×4.6 mm，10微米）上进行，该柱配有一个预柱（50 mm×4.6 mm，10微米，YWG-C18）。洗脱液为甲醇-水（30:70），流速为1.0 ml·min-1，在210 nm处进行紫外检测。血浆中的检测限为1.0微克·ml-1，校准曲线在5-1000微克·ml-1范围内呈线性（r = 0.9988）。该高效液相色谱法用于研究HMBA在兔体内的药代动力学。
