Department of Oral and Maxillofacial Surgery, Christian-Albrechts-University of Kiel, Kiel, Germany.
J Mater Sci Mater Med. 2010 Apr;21(4):1255-62. doi: 10.1007/s10856-009-3878-y. Epub 2010 Feb 7.
The aim of this study was to evaluate and compare the biocompatibility of computer-assisted designed (CAD) synthetic hydroxyapatite (HA) and tricalciumphosphate (TCP) blocks and natural bovine hydroxyapatite blocks for augmentations and endocultivation by supporting and promoting the proliferation of human periosteal cells. Human periosteum cells were cultured using an osteogenic medium consisting of Dulbecco's modified Eagle medium supplemented with fetal calf serum, Penicillin, Streptomycin and ascorbic acid at 37 degrees C with 5% CO(2). Three scaffolds were tested: 3D-printed HA, 3D-printed TCP and bovine HA. Cell vitality was assessed by Fluorescein Diacetate (FDA) and Propidium Iodide (PI) staining, biocompatibility with LDH, MTT, WST and BrdU tests, and scanning electron microscopy. Data were analyzed with ANOVAs.
After 24 h all samples showed viable periosteal cells, mixed with some dead cells for the bovine HA group and very few dead cells for the printed HA and TCP groups. The biocompatibility tests revealed that proliferation on all scaffolds after treatment with eluate was sometimes even higher than controls. Scanning electron microscopy showed that periosteal cells formed layers covering the surfaces of all scaffolds 7 days after seeding.
It can be concluded from our data that the tested materials are biocompatible for periosteal cells and thus can be used as scaffolds to augment bone using tissue engineering methods.
本研究旨在评估和比较计算机辅助设计（CAD）合成羟磷灰石（HA）和三钙磷酸盐（TCP）块与天然牛羟磷灰石块的生物相容性，通过支持和促进人骨膜细胞的增殖来增强和内生培养。人骨膜细胞在含有胎牛血清、青霉素、链霉素和抗坏血酸的改良杜尔贝科氏 Eagle 培养基中于 37 摄氏度、5%CO(2)下进行培养。测试了三种支架：3D 打印 HA、3D 打印 TCP 和牛 HA。细胞活力通过荧光素二乙酸酯（FDA）和碘化丙啶（PI）染色、LDH、MTT、WST 和 BrdU 测试以及扫描电子显微镜评估。数据采用 ANOVA 进行分析。
24 小时后，所有样本均显示有活力的骨膜细胞，与牛 HA 组的一些死细胞混合，而打印 HA 和 TCP 组的死细胞很少。生物相容性测试显示，在用洗脱液处理后，所有支架上的增殖有时甚至高于对照。扫描电子显微镜显示，接种后 7 天，骨膜细胞在所有支架表面形成覆盖层。
从我们的数据可以得出结论，测试的材料对骨膜细胞具有生物相容性，因此可用作组织工程方法增强骨的支架。
