Corner L A, Outteridge P M, Pearson W, Lepper A W
Int Arch Allergy Appl Immunol. 1976;52(1-4):3-14. doi: 10.1159/000231660.
Complete desensitization to tuberculin skin-testing (anergy) was produced in cattle by repeated intravenous injections of living BCG organisms into animals sensitized by a prior subcutaneous dose of BCG. Two levels of desensitization were produced; complete desensitization following 10 i.v. doses and partial desensitization following 5 i.v. doses of 100 mg BCG. Intradermal tuberculin testing at the end of the experiment stimulated the appearance of reactive blood lymphocytes in sensitized cattle as measured by in vitro 3H-thymidine uptake. The cattle which were partially desensitized showed this response but the completely desensitized cattle did not. Reactivity of blood lymphocytes in vitro to PHA and Brucella abortus antigen was not depressed in the anergic cattle. Serum antibody titres to BCG polysaccharide, PPD, or whole BCG organisms showed remarkably little change during the i.v. desensitizing injections of BCG. Differential blood leucocyte counts also remained within normal limits during this period. The production of MIF by blood lymphocytes from the anergic cattle appeared to be unimpaired. Using 3H-thymidine uptake by lymphocytes from sensitized cattle did not inhibit lymphocyte stimulation with tuberculin. It was concluded that the lack of tuberculin sensitized lymphocytes in the blood of anergic cattle may have been due to their removal from the recirculating pool and their continued suppression in lymphoid tissue.
通过向先前经皮下接种卡介苗致敏的牛重复静脉注射活卡介苗，使牛产生对结核菌素皮肤试验的完全脱敏（无反应性）。产生了两种脱敏水平：静脉注射10次100毫克卡介苗后完全脱敏，静脉注射5次后部分脱敏。实验结束时进行皮内结核菌素试验，通过体外3H-胸腺嘧啶核苷摄取量测定，刺激了致敏牛中反应性血液淋巴细胞的出现。部分脱敏的牛表现出这种反应，但完全脱敏的牛没有。无反应性牛血液淋巴细胞在体外对PHA和流产布鲁氏菌抗原的反应性并未降低。在静脉注射卡介苗进行脱敏注射期间，血清对卡介苗多糖、PPD或整个卡介苗生物体的抗体滴度变化极小。在此期间，血液白细胞分类计数也保持在正常范围内。无反应性牛血液淋巴细胞产生MIF的能力似乎未受损害。使用致敏牛淋巴细胞的3H-胸腺嘧啶核苷摄取量并不抑制结核菌素对淋巴细胞的刺激。得出的结论是，无反应性牛血液中缺乏结核菌素致敏淋巴细胞可能是由于它们从再循环池中被清除以及在淋巴组织中持续受到抑制。
