Tasaka K, Tsurukai T, Mio M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Agents Actions. 1994 Jun;41 Spec No:C106-7. doi: 10.1007/BF02007788.
HL-60 cells, treated under alkaline conditions (pH 7.6) or acidic conditions (pH 7.2) for 2 months, were stimulated with histamine for 7 days. From the morphological examination and cytochemical characterization, it became clear that one of the clones treated in acidic pH differentiated to neutrophils and the other clone treated in alkaline medium differentiated to eosinophils after histamine-stimulation. The growth curve reached a maximum 4 days after stimulation. By means of in situ hybridization, it has been shown that the mRNA of major basic protein increased after histamine treatment only in the eosinophilic subclone, starting 4 days after stimulation. From the present study, it is suggested that when HL-60 cells were cultured under different pH conditions, commitment of lineages to the direction of either eosinophils or neutrophils takes place. Histamine may potently stimulate the further differentiation of both eosinophilic and neutrophilic clones.
HL-60细胞在碱性条件（pH 7.6）或酸性条件（pH 7.2）下处理2个月后，用组胺刺激7天。通过形态学检查和细胞化学特征分析发现，组胺刺激后，在酸性pH条件下处理的一个克隆分化为中性粒细胞，而在碱性培养基中处理的另一个克隆分化为嗜酸性粒细胞。生长曲线在刺激后4天达到最大值。通过原位杂交表明，仅在嗜酸性亚克隆中，组胺处理后主要碱性蛋白的mRNA在刺激后4天开始增加。从本研究结果提示，当HL-60细胞在不同pH条件下培养时，细胞系向嗜酸性粒细胞或中性粒细胞方向的定向分化会发生。组胺可能有力地刺激嗜酸性和嗜中性克隆的进一步分化。
