Nakaya N, Tasaka K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Life Sci. 1988;42(9):999-1010. doi: 10.1016/0024-3205(88)90430-4.
The effect of histamine on granulocytic progenitor cells in murine bone marrow was studied in vitro. When bone marrow cells were cultured for three days with the drug, 10(-8) M to 10(-5) M of histamine stimulated differentiation and proliferation of myeloid precursor cells. Subsequently, the number of descendant cells, such as metamyelocytes and neutrophils, increased dose-dependently. Co-existence of equimolar H2 blockers such as cimetidine and ranitidine completely suppressed this effect of histamine, though this was not the case with an H1 blocker/histamine combination. Significant increase in 3H-thymidine incorporation was observed almost exclusively in myeloblasts, promyelocytes and myelocytes after exposure to histamine at concentrations higher than 10(-8) M. Also, selective incorporation of 3H-histamine into bone marrow cells was observed in myeloblasts and promyelocytes, but histamine incorporation was not influenced by the presence of either of histamine agonists or antagonists. While histamine, via H2 receptors, selectively increased the number of granulocytic colony forming units in culture (CFU-C), it had no such effect on macrophage colonies. Considering these findings, it was concluded that histamine promotes proliferation and differentiation of granulocytic myeloid cells via 1) H2 receptors in the CFU-C stage and 2) histamine receptors which are neither H1 nor H2 in the stages of myeloblast and promyelocyte differentiation.
体外研究了组胺对小鼠骨髓中粒细胞祖细胞的作用。当骨髓细胞与该药物培养三天时，10(-8)M至10(-5)M的组胺刺激了髓系前体细胞的分化和增殖。随后，晚幼粒细胞和中性粒细胞等后代细胞的数量呈剂量依赖性增加。西咪替丁和雷尼替丁等等摩尔H2阻滞剂的共存完全抑制了组胺的这种作用，尽管H1阻滞剂/组胺组合并非如此。在暴露于高于10(-8)M浓度的组胺后，几乎仅在原粒细胞、早幼粒细胞和中幼粒细胞中观察到3H-胸腺嘧啶核苷掺入显著增加。此外，在原粒细胞和早幼粒细胞中观察到3H-组胺选择性掺入骨髓细胞，但组胺掺入不受组胺激动剂或拮抗剂存在的影响。虽然组胺通过H2受体选择性增加培养物中粒细胞集落形成单位（CFU-C）的数量，但对巨噬细胞集落没有这种作用。考虑到这些发现，得出的结论是组胺通过1）CFU-C阶段的H2受体和2）原粒细胞和早幼粒细胞分化阶段既不是H1也不是H2的组胺受体促进粒细胞髓系细胞的增殖和分化。
