Corbel S, Dy M
CNRS URA 1461, Hôpital Necker, Paris, France.
Agents Actions. 1994 Jun;41 Spec No:C113-4. doi: 10.1007/BF02007791.
Based on previous evidence for a role of exogenous and endogenous histamine, we have examined whether this amine can effectively interact with hematopoietic progenitors. We show that tritiated histamine is retained preferentially by bone marrow cells as compared with peritoneal, thymic or spleen cells. Cells interacting with histamine copurify with progenitors in the low density bone marrow fraction. Among this cell population, 5% are labeled as assessed by autoradiography. The characteristics of histamine retention by these cells are consistent with active uptake rather than binding to a known receptor since (a) it is almost completely abrogated at 4 degrees C, by sodium azide or chloroquine, (b) histamine is internalized, and (c) relatively high concentrations of classical receptor antagonists are required to inhibit histamine retention by bone marrow cells.
基于先前有关外源性和内源性组胺作用的证据，我们研究了这种胺是否能有效地与造血祖细胞相互作用。我们发现，与腹膜、胸腺或脾脏细胞相比，骨髓细胞能优先保留氚标记的组胺。与组胺相互作用的细胞与低密度骨髓部分中的祖细胞共同纯化。在这个细胞群体中，通过放射自显影评估，有5%的细胞被标记。这些细胞保留组胺的特性与主动摄取一致，而不是与已知受体结合，因为：（a）在4℃时，叠氮化钠或氯喹几乎能完全消除这种作用；（b）组胺被内化；（c）需要相对高浓度的经典受体拮抗剂才能抑制骨髓细胞对组胺的保留。
