Dy M, Machavoine F, Lebel B, Ichikawa A, Gastinel L N, Schneider E
CNRS URA 1461, Hôpital Necker, Paris, France.
Biochem Biophys Res Commun. 1993 Apr 15;192(1):167-73. doi: 10.1006/bbrc.1993.1396.
Interleukin 3 (IL-3) is a potent stimulator of histamine production by cells from murine hematopoietic organs. We demonstrate herein that this phenomenon results from increased histidine decarboxylase (HDC: EC 4.1.1.22) activity in progenitor-enriched bone marrow cells (around 5% of the total bone marrow) isolated from the low density layers of a discontinuous Ficoll gradient. HDC levels are markedly enhanced after a 24 h incubation with IL-3 while a 4 h exposure results only in a slight activation. It results from increased expression of the mRNA coding for HDC, as assessed by Northern blot analysis after a 24 h incubation with IL-3. At the same time point and after a 4 h stimulation, we have evaluated the percentage of cells in this population which express HDC mRNA in response to IL-3, using in situ hybridization with the antisense riboprobe. We have thus established that enhanced HDC mRNA expression occurs in a small immature subset representing from 5 to 8% of the progenitor-enriched bone marrow cells.
白细胞介素3（IL-3）是一种由小鼠造血器官细胞产生组胺的强效刺激物。我们在此证明，这种现象是由于从不连续Ficoll梯度低密度层分离的富含祖细胞的骨髓细胞（约占骨髓总量的5%）中组氨酸脱羧酶（HDC：EC 4.1.1.22）活性增加所致。与IL-3孵育24小时后，HDC水平显著提高，而4小时的暴露仅导致轻微激活。这是由于编码HDC的mRNA表达增加，这是通过与IL-3孵育24小时后的Northern印迹分析评估得出的。在同一时间点以及4小时刺激后，我们使用反义核糖探针原位杂交技术评估了该群体中响应IL-3表达HDC mRNA的细胞百分比。因此我们确定，增强的HDC mRNA表达发生在一个小的未成熟亚群中，该亚群占富含祖细胞的骨髓细胞的5%至8%。
