Suwa Y, Inoue Y, Ohasi Y, Hayashi K
Department of Ophthalmology, Osaka Prefectural Habikino Hospital, Japan.
Nippon Ganka Gakkai Zasshi. 1994 Sep;98(9):820-4.
Cytomegalovirus (CMV) chorioretinitis is one of the commonest complications in immunocompromised hosts. However, the entire scope of ocular involvement during generalized CMV infection in immunocompromised hosts is yet to be elucidated. Balb/c nude mice were inoculated intraperitoneally with Smith strain of salivary gland passaged murine cytomegalovirus. Eight days after infection, they were sacrificed and the pathological features of virus spread were studied by immunofluorescence method. Subconjunctival tissues, corneal stroma, outer ocular muscles and occasional choroidal tissue are the site of positive fluorescence in the eye. At the same time, viral antigens were detected in the various organs such as spleen, liver, salivary glands, etc. Balb/c nude mice were injected intraperitoneally with silica three days and one day prior to virus infection to suppress their macrophages. The localization of viral antigens in the silica-treated mice was similar to that in untreated mice. When the peripheral blood obtained from the nude mice infected for 8 days was titrated for the virus, cell-free virus and blood-cell-associated virus was detected. These results indicated that cell-free virus is the major source of its spread.
巨细胞病毒（CMV）脉络膜视网膜炎是免疫功能低下宿主中最常见的并发症之一。然而，免疫功能低下宿主发生全身性CMV感染时眼部受累的全貌仍有待阐明。将唾液腺传代的小鼠巨细胞病毒史密斯株腹腔接种于Balb/c裸鼠。感染8天后，处死小鼠，采用免疫荧光法研究病毒传播的病理特征。结膜下组织、角膜基质、眼外肌及偶尔的脉络膜组织是眼部阳性荧光的部位。同时，在脾脏、肝脏、唾液腺等多种器官中检测到病毒抗原。在病毒感染前3天和1天，给Balb/c裸鼠腹腔注射二氧化硅以抑制其巨噬细胞。二氧化硅处理小鼠中病毒抗原的定位与未处理小鼠相似。对感染8天的裸鼠外周血进行病毒滴定，检测到游离病毒和血细胞相关病毒。这些结果表明，游离病毒是其传播的主要来源。
