Hayashi K, Suwa Y, Shimomura Y, Ohashi Y
Public Health Research Institute, Kobe, Japan.
J Med Virol. 1995 Dec;47(4):364-9. doi: 10.1002/jmv.1890470412.
Balb/C nude and C.B-17 SCID mice were inoculated with salivary gland passaged cytomegalovirus (SG-MCMV) intraperitoneally. Dissemination of the virus in the systemic and ocular tissues was studied by the direct immunofluorescence test, and the virus growth in each tissue was titrated in mouse embryonic fibroblasts. The mode of viral spread was assessed by inhibiting macrophage function by silica and administering polyclonal murine anti-MCMV antibody in the circulation. The virus first reached the eyelid, conjunctiva, and cornea. Subsequently, it spread in the outer ocular muscles and chorioretinal layer. Ocular tissues were involved as part of a generalized infection. Abrogation of macrophage function by silica did not affect the outcome of the viral distribution. Administration of antibody prior to and 3 days after the viral infection prevented virus dissemination. Ocular CMV infection occurred initially at the anterior segment of the eye in an immunocompromised host. Free virus, not macrophage-bound virus, disseminated via the bloodstream.
将Balb/C裸鼠和C.B-17 SCID小鼠经腹腔接种唾液腺传代巨细胞病毒（SG-MCMV）。通过直接免疫荧光试验研究病毒在全身和眼部组织中的播散情况，并在小鼠胚胎成纤维细胞中对各组织中的病毒生长进行滴定。通过二氧化硅抑制巨噬细胞功能并在循环中给予多克隆鼠抗MCMV抗体来评估病毒传播方式。病毒首先到达眼睑、结膜和角膜。随后，它在外眼肌和脉络膜视网膜层中扩散。眼部组织作为全身感染的一部分而受累。二氧化硅对巨噬细胞功能的消除并不影响病毒分布的结果。在病毒感染前及感染后3天给予抗体可阻止病毒播散。眼部巨细胞病毒感染最初发生在免疫受损宿主的眼前节。游离病毒而非与巨噬细胞结合的病毒通过血流播散。
