Ben-Ezra J M, Kornstein M J, Grimes M M, Krystal G
Department of Pathology, Virginia Commonwealth, University/Medical College of Virginia 23298-0662.
Am J Pathol. 1994 Nov;145(5):1036-40.
In our laboratory, we observed that a case of small cell carcinoma of the lung (SCLC) stained with a monoclonal antibody (Dako Corp., Bcl-2-124) against the Bcl-2 protein. To determine how common this reaction was, and whether it was specific for SCLC, we stained formalin-fixed, paraffin-embedded tissues from 23 cases of SCLC and 20 cases of squamous cell carcinoma of the lung for Bcl-2. Fifteen of the 23 SCLC cases stained positively for the Bcl-2 oncogene protein. In contrast, weak staining was observed in only three of the squamous cell carcinomas (chi 2-test; P = 0.001). In addition, four small cell carcinoma cell lines (H69, H146, H209, and WB) were tested by immunohistochemistry and flow cytometry for expression of this protein; all four were positive. In these and three other (H128, H432, and H510) SCLC lines, Northern blots of polyadenylated RNA revealed expression of the 6-kb Bcl-2 mRNA. Moreover, Western blots of extracts from these cell lines revealed the characteristic 26-kd band for Bcl-2 protein. In a single cell line, H82, which has previously been characterized as a variant SCLC, we failed to detect expression of the Bcl-2-specific mRNA and protein. We therefore conclude that most cases of SCLC of the lung express the Bcl-2 oncoprotein, which could play a role in the pathogenesis of this disease.
在我们实验室，我们观察到一例肺小细胞癌（SCLC）用抗Bcl-2蛋白的单克隆抗体（达科公司，Bcl-2-124）染色。为确定这种反应的常见程度以及它是否对SCLC具有特异性，我们对23例SCLC和20例肺鳞状细胞癌的福尔马林固定、石蜡包埋组织进行了Bcl-2染色。23例SCLC病例中有15例Bcl-2癌基因蛋白染色呈阳性。相比之下，仅在3例鳞状细胞癌中观察到弱阳性染色（卡方检验；P = 0.001）。此外，通过免疫组织化学和流式细胞术检测了4种小细胞癌细胞系（H69、H146、H209和WB）中该蛋白的表达；所有4种细胞系均呈阳性。在这些以及其他3种（H128、H432和H510）SCLC细胞系中，聚腺苷酸化RNA的Northern印迹显示有6-kb Bcl-2 mRNA的表达。此外，这些细胞系提取物的Western印迹显示出Bcl-2蛋白特征性的26-kd条带。在一个先前被鉴定为变异型SCLC的单细胞系H82中，我们未能检测到Bcl-2特异性mRNA和蛋白的表达。因此，我们得出结论，大多数肺SCLC病例表达Bcl-2癌蛋白，其可能在该疾病的发病机制中起作用。
