Lu Q L, Poulsom R, Wong L, Hanby A M
Histopathology Unit, Imperial Cancer Research Fund/Royal College of Surgeons of England, London, U.K.
J Pathol. 1993 Apr;169(4):431-7. doi: 10.1002/path.1711690408.
The B-cell leukaemia/lymphoma-2 (bcl-2) proto-oncogene is unusual as its product appears to provide survival advantage to B cells by blocking apoptosis. In this study, the expression of bcl-2 has been examined in normal non-haematopoietic tissues, embryos, and psoriatic skin by immunohistochemical staining. Bcl-2 protein expression is mainly observed in cell populations with a long life and/or proliferating ability such as duct cells in exocrine glands, basal keratinocytes, cells at the bottom of colon crypts, and neurons. In the skin of both adult and embryo and also embryonic kidney and cartilage, bcl-2 expression was observed in cells which were undergoing morphological transition from undifferentiated stem cells to committed precursor cells. The finding of bcl-2 expression in the terminal differentiated syncytial trophoblast, but not cytotrophoblast, and in some cells responsive to hormone stimulation such as in the endometrium and myometrium suggests that the gene expression may be related to hormone responsiveness. As no bcl-2 localization was seen in the benign hyperproliferative skin condition psoriasis, this does not suggest a straight-forward link to proliferation. These observations support the view that the bcl-2 gene may have an important role in cell development, maturation, and the path to terminal differentiation.
B 细胞白血病/淋巴瘤-2（bcl-2）原癌基因不同寻常，因为其产物似乎通过阻止细胞凋亡为 B 细胞提供生存优势。在本研究中，通过免疫组织化学染色检测了 bcl-2 在正常非造血组织、胚胎和银屑病皮肤中的表达。Bcl-2 蛋白表达主要见于具有长寿命和/或增殖能力的细胞群体，如外分泌腺的导管细胞、基底角质形成细胞、结肠隐窝底部的细胞和神经元。在成年和胚胎皮肤以及胚胎肾和软骨中，在正经历从未分化干细胞向定向前体细胞形态转变的细胞中观察到了 bcl-2 表达。在终末分化的合体滋养层而非细胞滋养层以及一些对激素刺激有反应的细胞（如子宫内膜和子宫肌层中的细胞）中发现 bcl-2 表达，这表明基因表达可能与激素反应性有关。由于在良性增殖性皮肤病银屑病中未观察到 bcl-2 定位，这并不表明其与增殖有直接联系。这些观察结果支持这样一种观点，即 bcl-2 基因可能在细胞发育、成熟以及终末分化途径中起重要作用。
