Simonitsch I, Volc-Platzer B, Mosberger I, Radaszkiewicz T
Institute of Clinical Pathology, University of Vienna Medical School, Austria.
Am J Pathol. 1994 Nov;145(5):1148-58.
Considering that integrins may play a major role in the localization in distinct biological features as well as in the dissemination of several types of lymphomas, we studied the expression of the monoclonal antibody HML-1-defined alpha E beta 7 integrin (CD103) in the clinically and histologically determined stages of 53 mycosis fungoides (MF) skin biopsies and in 16 affected lymph nodes. Immunoperoxidase staining revealed HML-1 immunoreactivity with T cells in the early stages of disease (patch and plaque stage MF). HML-1 expression was more pronounced on infiltrating epidermal than on dermal T cells. In contrast to early stages, tumor stage MF and lymph nodes affected in the course of cutaneous T cell lymphoma were HML-1 negative. We found a strong association between HML-1 expression, epidermotropism of infiltrating T cells, and the stage of disease. We provide evidence that: 1) the loss of the HML-1 antigen on T cells in MF is a marker of poor prognosis and 2) because the HML-1 antigen is selectively expressed on T lymphocytes of epithelial sites such as gut and skin, our results are compatible with the view that alpha E beta 7 integrins perform homing receptor functions for epitheliotropic T cells.
鉴于整合素可能在不同生物学特征的定位以及几种类型淋巴瘤的播散中起主要作用，我们研究了单克隆抗体HML-1所定义的αEβ7整合素（CD103）在53例蕈样肉芽肿（MF）皮肤活检及16例受累淋巴结的临床和组织学确定分期中的表达情况。免疫过氧化物酶染色显示，在疾病早期（斑块期和斑片期MF），HML-1与T细胞呈现免疫反应性。HML-1在浸润表皮的T细胞上的表达比在真皮T细胞上更为明显。与疾病早期不同，肿瘤期MF以及皮肤T细胞淋巴瘤病程中受累的淋巴结HML-1呈阴性。我们发现HML-1表达、浸润T细胞的亲表皮性与疾病分期之间存在密切关联。我们提供的证据表明：1）MF中T细胞上HML-1抗原的缺失是预后不良的标志物；2）由于HML-1抗原在肠道和皮肤等上皮部位的T淋巴细胞上选择性表达，我们的结果与αEβ7整合素对亲上皮性T细胞发挥归巢受体功能的观点相符。
