Deutsch J, Grange E, Rapoport S I, Purdon A D
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892.
Anal Biochem. 1994 Aug 1;220(2):321-3. doi: 10.1006/abio.1994.1344.
Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.
长链脂酰辅酶A是脂肪酸氧化和磷脂代谢中的重要中间体。为了对脑脂酰辅酶A进行定量分析，并避免因高脑脂酰辅酶A水解酶活性导致的分解，开发了一种快速高效的分析方法用于脂酰辅酶A的分离和测定。该分析包括使用寡核苷酸纯化柱进行固相萃取以及使用合成内标进行高效液相色谱测量。大鼠脑中的浓度估计为：油酰辅酶A（11.0纳摩尔/克）、棕榈酰辅酶A（6.0纳摩尔/克）、硬脂酰辅酶A（4.0纳摩尔/克）以及亚油酰辅酶A和花生四烯酰辅酶A（2.0纳摩尔/克），脑总浓度为23纳摩尔/克。
