Ferreira-Dias G, Nequin L G, King S S
Department of Physiology, Southern Illinois University, Carbondale 62901-6512.
Am J Vet Res. 1994 Aug;55(8):1060-5.
Pathologic changes in the endometrium of mares may be rated according to Kenney's method of classification. Category I endometrium contains healthy tissue with no or few widely scattered pathologic changes. At the opposite end, severe widespread pathologic changes are associated with category III. Uterine biopsy specimens were collected aseptically from 16 mares during the estrous and diestrous stages of the cycle. Pathologic changes were evaluated, using light microscopy, and endometrium was classified as Kenney's category I, II, or III. Endometrial tissue of category I (n = 5 mares in estrus; n = 3 in diestrus); category II (n = 3 in estrus; n = 4 in diestrus), and category III (n = 4 in estrus; n = 4 in diestrus) were processed for scanning electron microscopy (SEM). All specimens were fixed immediately after biopsy because it was found that numerous bleb-like projections were formed when fixation was delayed. Category I endometrium had normal glands, and fibrotic tissue was not observed by light microscopy. Scanning electron microscopy revealed numerous hexagonally shaped cells that were covered with many microvilli. Ciliated cells also were observed, and they contained long healthy cilia. Category II endometrium had 2 to 4 nests surrounded by collagen fibers. Of the 4 specimens, 3 had moderate leukocyte infiltration (59 +/- 14.8 WBC/4 high-power fields [450 x]). Scanning electron microscopy revealed some inflammatory changes with slight swelling of the cell surface. Several cells in category II endometrium lacked microvilli, but they were interdispersed among many healthy hexagonal cells. Many nests were seen in category III tissue, and 2 specimens had severe infiltration of WBC (232,264 cells/4 fields).(ABSTRACT TRUNCATED AT 250 WORDS)
母马子宫内膜的病理变化可根据肯尼的分类方法进行分级。I类子宫内膜含有健康组织，无或仅有少量广泛散在的病理变化。与之相反，严重的广泛病理变化与III类相关。在发情周期和发情间期，从16匹母马中无菌采集子宫活检标本。使用光学显微镜评估病理变化，并将子宫内膜分类为肯尼的I类、II类或III类。对I类（发情期5匹母马；发情间期3匹）、II类（发情期3匹母马；发情间期4匹）和III类（发情期4匹母马；发情间期4匹）的子宫内膜组织进行扫描电子显微镜（SEM）检查。所有标本在活检后立即固定，因为发现固定延迟时会形成许多泡状突起。I类子宫内膜有正常腺体，光学显微镜下未观察到纤维化组织。扫描电子显微镜显示有许多六边形细胞，表面覆盖着许多微绒毛。还观察到纤毛细胞，它们含有长而健康的纤毛。II类子宫内膜有2至4个被胶原纤维包围的巢。在4个标本中，3个有中度白细胞浸润（59±14.8个白细胞/4个高倍视野[450×]）。扫描电子显微镜显示有一些炎症变化，细胞表面略有肿胀。II类子宫内膜中的一些细胞没有微绒毛，但它们散布在许多健康的六边形细胞之间。在III类组织中可见许多巢，2个标本有严重的白细胞浸润（232,264个细胞/4个视野）。（摘要截短于250字）
