Ferreira-Dias G M, Nequin L G, King S S
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901, USA.
Am J Vet Res. 1999 Jan;60(1):49-55.
To evaluate whether the pathologic changes observed by light microscopy in endometrium of categories II and III were reflected by cellular changes and to describe differences in the endometrial cell ultrastructure during estrus and diestrus.
18 healthy mares.
Endometrial tissues biopsied during the physiologic breeding season were categorized, using light microscopy, and were studied, using transmission electron microscopy (TEM).
Using TEM, glycogen granules were associated with giant mitochondria for all endometrial types during diestrus. Development of rough endoplasmic reticulum (RER) and Golgi apparatus suggested protein synthesis in the endometrial glands during diestrus. TEM did not reveal major ultrastructural differences, between endometrium of categories I and II. This was unlike differences identified by light microscopy. The most extensive pathologic changes were seen in category-III tissue (TEM and light microscopy). Category-III endometria had a large number of light cells with more degenerative structures and fewer organelles, and lacked cilia in the lumen of the glands. This tissue had extensive fibrotic tissue in the lamina propria and many inflammatory cells in most tissue layers.
The severe ultrastructural changes may be one of the many factors decreasing the fertility of mares with category-III, compared with category-1 and -2, endometrium.
评估通过光学显微镜观察到的II类和III类子宫内膜的病理变化是否由细胞变化所反映，并描述发情期和间情期子宫内膜细胞超微结构的差异。
18匹健康母马。
在生理繁殖季节采集的子宫内膜组织，先用光学显微镜进行分类，再用透射电子显微镜（TEM）进行研究。
使用透射电子显微镜观察发现，间情期所有类型子宫内膜的糖原颗粒均与巨大线粒体相关。粗面内质网（RER）和高尔基体的发育表明间情期子宫内膜腺体中有蛋白质合成。透射电子显微镜未显示I类和II类子宫内膜之间存在主要的超微结构差异。这与光学显微镜所发现的差异不同。III类组织（透射电子显微镜和光学显微镜观察）出现的病理变化最为广泛。III类子宫内膜有大量亮细胞，其退化结构更多，细胞器更少，腺腔内缺乏纤毛。该组织固有层有广泛的纤维化组织，大多数组织层中有许多炎性细胞。
与I类和II类子宫内膜的母马相比，严重的超微结构变化可能是导致III类子宫内膜母马生育力下降的众多因素之一。
