John W G, Edwards R, Price C P
Department of Clinical Biochemistry, Royal London Hospital, Whitechapel, UK.
Ann Clin Biochem. 1994 Jul;31 ( Pt 4):367-70. doi: 10.1177/000456329403100411.
The DCA 2000 clinical analyser for the measurement of haemoglobin A1c was evaluated for analytical quality. The analyser, which utilises inhibition of latex agglutination immunoassay, demonstrated good within-batch (1.9-3.1% CV) and between-batch (2.2% CV) imprecision, and was not affected by haemoglobin concentration. The analyser was linear throughout the analytical range, and was found to correlate well with agar electroendosmosis (r = 0.93), affinity chromatography (r = 0.97), HPLC (r = 0.90) and EIA (r = 0.98). The analyser was found to give reliable analytical results, and with its ease of use, will provide the diabetologist with HbA1c results in the clinic; although an analysis time of 9 min will limit sample throughput.
对用于测量糖化血红蛋白的DCA 2000临床分析仪进行了分析质量评估。该分析仪采用乳胶凝集免疫抑制法，批内不精密度良好（变异系数为1.9 - 3.1%），批间不精密度为2.2%变异系数，且不受血红蛋白浓度影响。该分析仪在整个分析范围内呈线性，并且发现与琼脂电渗（r = 0.93）、亲和色谱法（r = 0.97）、高效液相色谱法（r = 0.90）和酶免疫测定法（r = 0.98）相关性良好。该分析仪能给出可靠的分析结果，且使用方便，将为临床糖尿病专家提供糖化血红蛋白的检测结果；不过9分钟的分析时间会限制样本通量。
