Pasqualini J R, Maloche C, Maroni M, Chetrite G
C.N.R.S. Steroid Hormone Research Unit, Foundation for Hormone Research, Paris, France.
Anticancer Res. 1994 Jul-Aug;14(4A):1589-93.
Using reverse transcriptase polymerase chain reaction amplification it was possible to detect the presence of oestrogen sulphatase mRNA in different hormone-dependent (MCF-7, T-47D) and hormone-independent (MDA-MB-231, MDA-MB-468) mammary cancer cell lines. The expression of this mRNA is significantly higher in T-47D and MDA-MB-231 than in the other cell lines, and a correlation of this expression with the enzymatic activities was observed. The progestagen Promegestone (R-5020) can significantly decrease the mRNA of the sulphatase in MCF-7 cells. As this progestagen can also inhibit the enzyme itself in the same mammary cancer cell line, it is suggested that for the decrease in the sulphatase activity not only the effect on the enzyme, but also the effect on transcriptional factor(s) which express this enzyme are involved. The present data not only contribute to the knowledge of the mechanism of the sulphatase activity, but also can open new possibilities in breast cancer treatment.
通过逆转录聚合酶链反应扩增，能够在不同的激素依赖性（MCF - 7、T - 47D）和激素非依赖性（MDA - MB - 231、MDA - MB - 468）乳腺癌细胞系中检测到雌激素硫酸酯酶mRNA的存在。该mRNA在T - 47D和MDA - MB - 231中的表达显著高于其他细胞系，并且观察到这种表达与酶活性之间存在相关性。孕激素普美孕酮（R - 5020）可显著降低MCF - 7细胞中硫酸酯酶的mRNA。由于这种孕激素在同一乳腺癌细胞系中也能抑制该酶本身，因此表明硫酸酯酶活性的降低不仅涉及对酶的作用，还涉及对表达该酶的转录因子的作用。目前的数据不仅有助于了解硫酸酯酶活性的机制，还可为乳腺癌治疗开辟新的可能性。
