Tateno M, Ihara K, Mukohata Y
Department of Biology, School of Science, Nagoya University, Japan.
Arch Biochem Biophys. 1994 Nov 15;315(1):127-32. doi: 10.1006/abbi.1994.1480.
Extreme halophiles newly collected from Argentine salt flats were characterized, in one of which, Haloarcula (sp. arg-1), light-driven retinal protein ion pumps were found. The proton pump, cruxrhodopsin-1, shows amino acid sequence homologies of 52% to bacteriorhodopsin and 48% to archaerhodopsin-1. The anion pump, cruxhalorhodopsin-1, identified partially as a 394bp polymerase chain reaction product, shows homologies of 70% to halorhodopsin, and 72% to pharaonis halorhodopsin. The ion pumps (and possibly sensors still to be found) in Haloarcula sp. arg-1, which constitute the cruxrhodopsin-1 family, are distinct from the bacteriorhodopsin and the archaerhodopsin families/tribes.
对从阿根廷盐沼新采集的嗜盐菌进行了特性分析，其中一种名为嗜盐碱杆菌属（菌株arg-1）的嗜盐菌中发现了光驱动的视黄醛蛋白离子泵。质子泵cruxrhodopsin-1与细菌视紫红质的氨基酸序列同源性为52%，与古菌视紫红质-1的同源性为48%。阴离子泵cruxhalorhodopsin-1经部分鉴定为394bp聚合酶链反应产物，与盐视紫红质的同源性为70%，与法老盐视紫红质的同源性为72%。嗜盐碱杆菌属菌株arg-1中的离子泵（以及可能仍有待发现的传感器）构成了cruxrhodopsin-1家族，与细菌视紫红质和古菌视紫红质家族/类群不同。
