Kitajima T, Hirayama J, Ihara K, Sugiyama Y, Kamo N, Mukohata Y
Department of Biology, School of Science, Nagoya University, Japan.
Biochem Biophys Res Commun. 1996 Mar 18;220(2):341-5. doi: 10.1006/bbrc.1996.0407.
New bacterial rhodopsins of the cruxrhodopsin (cR) tribe were identified in a type strain Haloarcula vallismortis. The genes encoding a bacteriorhodopsin-like ion pump (named cR-3), a halorhodopsin-like ion pump (chR-3) and a sensor rhodopsin (csR-3) were cloned and sequenced. Together with the data for vsRII (Seidel et al., Proc. Natl. Acad. Sci. USA 92, 3036-3040 (1995); cpR-3 in our notation), the primary structures of a set of four rhodopsins are now all known only in this species. They are separated by almost the same distances in homology, suggesting that they have derived from a single ancestral rhodopsin. The degree of conservation in the amino acid sequence of each helix showed that helices C and G are relatively well conserved in all rhodopsins, whereas helices DEF are conserved especially in sensor rhodopsin-I, possibly because these helices are needed for interaction with the transducer protein (Htr).
在嗜盐碱嗜盐菌（Haloarcula vallismortis）的模式菌株中鉴定出了十字视紫红质（cR）族的新型细菌视紫红质。编码一种细菌视紫红质样离子泵（命名为cR-3）、一种嗜盐视紫红质样离子泵（chR-3）和一种传感视紫红质（csR-3）的基因被克隆并测序。连同视紫红质II（vsRII）的数据（Seidel等人，《美国国家科学院院刊》92，3036 - 3040（1995）；我们标记法中的cpR-3），现在仅在该物种中知道一组四种视紫红质的一级结构。它们在同源性上被几乎相同的距离隔开，表明它们源自单一的祖先视紫红质。每个螺旋氨基酸序列中的保守程度表明，螺旋C和G在所有视紫红质中相对保守，而螺旋DEF尤其在传感视紫红质-I中保守，这可能是因为这些螺旋是与转导蛋白（Htr）相互作用所必需的。
