Scott T M, Chafe L
Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Artery. 1994;21(1):51-62.
The distribution of the ability of perivascular peptidergic innervation to influence acetylcholine-induced relaxation in the arterial bed has been investigated using the superior mesenteric arterial bed of the rat. The superior mesenteric artery was denervated by freezing, under pentobarbital anaesthesia (40 mg/kg i.p.), at one of two different points and the animals allowed to survive for 7, 14, 21 or 28 days following freezing. This produced a range of denervation along the superior mesenteric artery and its branches. The ability to respond to acetylcholine in an endothelium-dependent fashion was determined pharmacologically in isolated perfused superior mesenteric artery preparations. The extent of the denervation was determined by immunohistochemistry. It was found that the ability of the arterial bed to relax in a concentration-dependent manner to increasing concentrations of acetylcholine was little altered by denervation of the superior mesenteric artery alone. However when a short piece of the superior mesenteric artery and its primary branches were denervated the response to acetylcholine was reduced. It is concluded that the innervation of the primary branches of the mesenteric artery, the jejunal and ileal branches, contributes to the ability of acetylcholine to cause endothelium-dependent relaxation.
利用大鼠肠系膜上动脉床，研究了血管周围肽能神经支配对动脉床中乙酰胆碱诱导舒张能力的影响分布。在戊巴比妥麻醉（腹腔注射40mg/kg）下，于两个不同点之一对肠系膜上动脉进行冷冻去神经支配，并使动物在冷冻后存活7、14、21或28天。这在肠系膜上动脉及其分支上产生了一系列去神经支配。通过药理学方法在离体灌注的肠系膜上动脉制备物中测定以内皮依赖性方式对乙酰胆碱作出反应的能力。通过免疫组织化学确定去神经支配的程度。结果发现，仅肠系膜上动脉去神经支配对动脉床以浓度依赖性方式对乙酰胆碱浓度增加作出舒张反应的能力影响很小。然而，当肠系膜上动脉及其主要分支的一小段去神经支配时，对乙酰胆碱的反应减弱。得出的结论是，肠系膜动脉的主要分支、空肠和回肠分支的神经支配有助于乙酰胆碱引起内皮依赖性舒张的能力。
