Opara O O, Carr L E, Tate C R, Miller R G, Mallinson E T, Stewart L E, Joseph S W
Department of Microbiology, University of Maryland, College Park 20742.
Avian Dis. 1994 Apr-Jun;38(2):293-6.
The drag-swab Salmonella screening technique was evaluated using less expensive alternatives to double-strength skim milk (2 x SM) as a saturating medium for drag swabs. Ten pre-determined Salmonella-positive poultry houses were studied. In the first phase, Salmonella screening efficiency of drag swabs impregnated with 2 x SM and commercially available canned Carnation evaporated skim milk (CESM) were compared. Results showed CESM to be a less efficient alternative. In the second phase of the study, the Salmonella screening efficiency of drag swabs impregnated with 2% buffered peptone water (BPW), physiological saline (PS), and distilled water (DW) were evaluated along with an unimpregnated drag swab (dry drag swab) (DD) as possible alternatives to 2 x SM. The efficiency of Salmonella detection using various impregnation treatments were in the following order: 2 x SM > PS > BPW > DW > DD.
使用比双倍强度脱脂乳(2xSM)成本更低的替代品作为拖拭棒的饱和培养基,对拖拭棒沙门氏菌筛查技术进行了评估。研究了10个预先确定为沙门氏菌阳性的禽舍。在第一阶段,比较了用2xSM浸渍的拖拭棒和市售罐装康乃馨脱脂奶粉(CESM)的沙门氏菌筛查效率。结果表明,CESM是一种效率较低的替代品。在研究的第二阶段,评估了用2%缓冲蛋白胨水(BPW)、生理盐水(PS)和蒸馏水(DW)浸渍的拖拭棒以及未浸渍的拖拭棒(干拖拭棒)(DD)作为2xSM的可能替代品的沙门氏菌筛查效率。使用各种浸渍处理检测沙门氏菌的效率顺序如下:2xSM>PS>BPW>DW>DD。