Thompson H S, Scordilis S P
Department of Biological Sciences, Smith College, Northampton, MA 01063.
Biochem Biophys Res Commun. 1994 Nov 15;204(3):1193-8. doi: 10.1006/bbrc.1994.2589.
Changes in ubiquitin levels were characterized in human biceps muscle following high-force eccentric exercise. Volunteers performed damaging eccentric-isokinetic actions of the biceps muscle with the non-dominant arm. Protein extracts of biopsy tissue samples taken two days post-exercise were run on SDS polyacrylamide gels, analyzed densitometrically and revealed a 64% higher level of a protein band at 12 kD. New monoclonal immunoblotting techniques identified the band as free ubiquitin. On these blots, free ubiquitin increased in the exercised sample by 55% over the control, and ubiquitin conjugates of varying molecular weights follow a similar pattern. The changes seen in both free and conjugated ubiquitin suggest that their increases are involved in the response to exercise-induced muscle damage.
在高强度离心运动后人肱二头肌中泛素水平的变化得到了表征。志愿者用非优势手臂进行肱二头肌的损伤性离心等动运动。运动后两天采集的活检组织样本的蛋白质提取物在SDS聚丙烯酰胺凝胶上进行电泳，进行光密度分析，结果显示在12 kD处有一条蛋白带的水平高出64%。新的单克隆免疫印迹技术将该条带鉴定为游离泛素。在这些印迹上，运动样本中的游离泛素比对照增加了55%，不同分子量的泛素缀合物也呈现类似的模式。游离和缀合泛素中观察到的变化表明，它们的增加参与了对运动诱导的肌肉损伤的反应。
