Guillamón J M, Barrio E, Huerta T, Querol A
Departamento de Microbiología, Facultad de Ciencias Biológicas, Universitat de València, Spain.
Int J Syst Bacteriol. 1994 Oct;44(4):708-14. doi: 10.1099/00207713-44-4-708.
Several strains of the four sibling species of the genus Saccharomyces (S. bayanus, S. cerevisiae, S. paradoxus, and S. pastorianus) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as AluI, DdeI, HinfI, and RsaI) made it possible to differentiate each species. S. cerevisiae and S. paradoxus presented a greater number of large fragments than S. pastorianus and S. bayanus with all the assay enzymes. With AluI and DdeI, species-specific bands clearly permitted differentiation between S. pastorianus and S. bayanus. To test the resolution of this method, wild Saccharomyces strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping.
利用一种快速且简单的线粒体DNA限制性分析方法，对酵母属（巴氏酵母、酿酒酵母、奇异酵母和 pastorianus酵母）的四个姊妹种的多个菌株进行了特征分析。用四切点核酸内切酶（如AluI、DdeI、HinfI和RsaI）获得的图谱使得区分每个物种成为可能。在所有检测酶中，酿酒酵母和奇异酵母呈现出比pastorianus酵母和巴氏酵母更多的大片段。使用AluI和DdeI时，物种特异性条带清晰地允许区分pastorianus酵母和巴氏酵母。为了测试该方法的分辨率，对野生酵母菌株进行了分析。通过电泳核型分析证实了通过这种快速方法将这些菌株正确归类到已知分类单元。
