巴氏酵母变种葡萄汁酵母的线粒体DNA多态性

Mitochondrial DNA polymorphism of the yeast Saccharomyces bayanus var. uvarum.

作者信息

Naumova E S, Naumov G I, Barrio E, Querol A

机构信息

State Research Institute for Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyipmezd I, Moscow 117545, Russia.

出版信息

Mikrobiologiia. 2010 Jul-Aug;79(4):543-50.

PMID:21058508

Abstract

Genetic relationships among forty-one strains of Saccharomyces bayanus var. uvarum isolated in different wine regions of Europe and four wild isolates were investigated by restriction analysis (RFLP) of mitochondrial DNA (mtDNA) with four restriction endonucleases, AluI, DdeI, HinfI and RsaI. No clear correlation between origin and source of isolation of S. bayanus var. uvarum strains and their mtDNA restriction profiles was found. On the whole, the mtDNA of S. bayanus var. uvarum is much less polymorphic than that of S. cerevisiae. This observation is in good agreement with results obtained by electrophoretic karyotyping. Unlike wine S cerevisiae, strains of S. bayanus var. uvarum display a low level of chromosome length polymorphism.

摘要

通过使用四种限制性内切酶（AluI、DdeI、HinfI和RsaI）对线粒体DNA（mtDNA）进行限制性分析（RFLP），研究了在欧洲不同葡萄酒产区分离出的41株巴氏酵母葡萄变种菌株与4株野生分离株之间的遗传关系。未发现巴氏酵母葡萄变种菌株的来源和分离源与其mtDNA限制性图谱之间存在明显相关性。总体而言，巴氏酵母葡萄变种的mtDNA多态性远低于酿酒酵母。这一观察结果与通过电泳核型分析获得的结果高度一致。与葡萄酒酿酒酵母不同，巴氏酵母葡萄变种菌株的染色体长度多态性水平较低。