胃癌中DNA含量的定量测量；流式细胞术和视频图像分析。

Quantitative measurement of DNA content in gastric carcinoma; flow cytometry and video image analysis.

作者信息

Brito M J, Rosa J C, Thomas V A, Filipe M I

机构信息

Department of Histopathology, Sta Marta Hospital, Lisbon, Portugal.

出版信息

Anal Cell Pathol. 1994 Jul;7(1):1-9.

PMID:7981132

Abstract

The DNA content of 48 gastric carcinomas from archival material was analysed by static and flow cytometry. By image analysis 81.3% of the tumours were aneuploid and ploidy was related to stage (P = 0.024) and lymph node metastasis. A trend for better survival (> 12 months) was observed in patients with diploid tumours (P = 0.058). The mean 5c exceeding rate (5cER) was significantly related to tumour stage (P < 0.05) and patient's survival (P = 0.018). In contrast, by flow cytometry only 43.7% of these tumours were aneuploid and these were more often associated with lymph node metastasis (59.3%) but no relationship was observed with any other parameters or patient's survival. In this series, image analysis appears to be more sensitive than flow cytometry in detecting small aneuploid populations. It may give additional prognostic information. It is, however, a time-consuming technique.

摘要

采用静态和流式细胞术分析了取自存档材料的48例胃癌的DNA含量。通过图像分析，81.3%的肿瘤为非整倍体，并且倍性与分期（P = 0.024）及淋巴结转移相关。在二倍体肿瘤患者中观察到生存时间延长（> 12个月）的趋势（P = 0.058）。平均5c超率（5cER）与肿瘤分期（P < 0.05）及患者生存情况（P = 0.018）显著相关。相比之下，通过流式细胞术检测，这些肿瘤中只有43.7%为非整倍体，且这些肿瘤更常伴有淋巴结转移（59.3%），但未观察到与任何其他参数或患者生存情况的相关性。在本研究系列中，图像分析在检测小的非整倍体群体方面似乎比流式细胞术更敏感。它可能提供额外的预后信息。然而，这是一项耗时的技术。