Posho L, Darcy-Vrillon B, Morel M T, Cherbuy C, Blachier F, Duée P H
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, CRJ, Jouy-en-Josas, France.
Biochim Biophys Acta. 1994 Nov 10;1224(2):213-20. doi: 10.1016/0167-4889(94)90193-7.
The objective of the present work was to identify the regulatory step(s) in the post-natal development of a high glycolytic capacity previously evidenced in newborn pig enterocytes (Darcy-Vrillon et al. (1994) Pediat. Res., 36, 175-181. Glucose entry via the Na+/glucose cotransporter, estimated by the uptake of the non-metabolizable analogue methyl alpha-D-[U-14C]glucopyranoside, slightly decreased between birth and 2 days of sucking. The flux of glucose metabolized into the pentose cycle pathway slightly increased but could not account for the 3-fold increase observed in the glycolytic capacity. Whereas the maximal activity of 6-phosphofructo-1-kinase did not change between stages, there was a significant increase in hexokinase activity as well as in the flux of glucose phosphorylated. These findings suggest that the stimulation of glucose phosphorylation through hexokinase is the key event leading to an increased glycolytic capacity of small intestinal cells at the onset of sucking.
本研究的目的是确定新生猪小肠上皮细胞中先前已证实的高糖酵解能力在出生后发育过程中的调控步骤（Darcy-Vrillon等人，(1994)《儿科研究》，36, 175 - 181）。通过摄取不可代谢的类似物α-D-[U-14C]吡喃葡萄糖苷来估计经Na⁺/葡萄糖共转运体的葡萄糖摄取，在出生至吸吮2天之间略有下降。进入戊糖循环途径的葡萄糖通量略有增加，但无法解释糖酵解能力中观察到的3倍增长。虽然6-磷酸果糖-1-激酶的最大活性在各阶段之间没有变化，但己糖激酶活性以及葡萄糖磷酸化通量有显著增加。这些发现表明，通过己糖激酶刺激葡萄糖磷酸化是导致吸吮开始时小肠细胞糖酵解能力增加的关键事件。
