Novikov V V, Metlina A L, Poglazov B F
Department of Functional Biochemistry of Biopolymers, A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Russia.
Biochem Mol Biol Int. 1994 Jul;33(4):723-8.
Optical properties of intact flagellin and of modified-on-tyrosine flagellin incapable of self-assembly have been studied by the circular dichroism (CD) method. The CD spectra of both flagellins do not change and virtually coincide within the pH range from 2.9 to 10.0. In the presence of polyethylene glycol (PEG) and ammonium sulfate which accelerate flagellin polymerization, the character of the CD spectra changes and depends on pH. The increase in the PEG content to 20% and that in the ammonium sulfate content to 1 M over the pH range from 4.3 to 10.0 results in a significant rise of the molar ellipticity at 222 nm ([theta]222) of both flagellins. However, [theta]222 does not reach values typical for bacterial flagella. The results obtained are discussed with respect to conformational changes in the flagellin molecule during polymerization.
通过圆二色性（CD）方法研究了完整鞭毛蛋白以及不能自组装的酪氨酸修饰鞭毛蛋白的光学性质。两种鞭毛蛋白的CD光谱在pH值2.9至10.0范围内均无变化且几乎重合。在聚乙二醇（PEG）和硫酸铵存在下，它们会加速鞭毛蛋白聚合，此时CD光谱的特征会发生变化且取决于pH值。在pH值4.3至10.0范围内，将PEG含量增加至20%以及将硫酸铵含量增加至1 M，会导致两种鞭毛蛋白在222 nm处的摩尔椭圆率（[θ]222）显著升高。然而，[θ]222并未达到细菌鞭毛的典型值。结合聚合过程中鞭毛蛋白分子的构象变化对所得结果进行了讨论。
