通过毛细管电泳分析人淋巴瘤细胞中的核苷酸库。

Analysis of nucleotide pools in human lymphoma cells by capillary electrophoresis.

作者信息

Shao X, O'Neill K, Zhao Z, Anderson S, Malik A, Lee M

机构信息

Department of Microbiology, Brigham Young University, Provo, UT 84602.

出版信息

J Chromatogr A. 1994 Oct 7;680(2):463-8. doi: 10.1016/0021-9673(94)85144-1.

DOI:10.1016/0021-9673(94)85144-1

PMID:7981827

Abstract

Capillary electrophoresis is applied to determine nucleotide pool levels in human Burkitt lymphoma cells. The analysis was performed on a 65 cm x 50 microns I.D. Ucon-coated column with on-column UV detector. The method requires only nanoliters of sample and a simple sample preparation procedure. Over 12 nucleotides were separated and quantitated with high resolution and reproducibility. The whole capillary electrophoretic separation time was only 35 min. These results demonstrate that capillary electrophoresis provides a useful and easy way to analyze nucleotide pools in cells.

摘要

应用毛细管电泳法测定人伯基特淋巴瘤细胞中的核苷酸池水平。分析在一根内径50微米、长65厘米的涂有Ucon的柱上进行，配有柱上紫外检测器。该方法仅需纳升样品及简单的样品制备程序。超过12种核苷酸得以高分辨率和可重复性地分离与定量。整个毛细管电泳分离时间仅为35分钟。这些结果表明，毛细管电泳为分析细胞中的核苷酸池提供了一种有用且简便的方法。