Madamba-Tan L S, Strasters J K, Khaledi M G
Department of Chemistry, North Carolina State University, Raleigh 27695-8204.
J Chromatogr A. 1994 Oct 21;683(2):321-34. doi: 10.1016/0021-9673(94)00450-1.
Gradient elution in micellar liquid chromatography (MLC) is discussed. On the basis of the gradient elution theory, first developed by Snyder, equations were derived for the prediction of gradient retention times in micelle concentration gradient from isocratic data. Likewise, partition coefficients into micelles and stationary phase, and subsequently isocratic retention at different micelle concentrations can be estimated from two gradient runs. However, more studies need to be done to achieve better agreement between isocratic and gradient data. The equations will be useful for efficient development of practical separations by MLC.
讨论了胶束液相色谱法（MLC）中的梯度洗脱。基于最初由斯奈德提出的梯度洗脱理论，推导了用于从等度数据预测胶束浓度梯度中梯度保留时间的方程。同样，可以通过两次梯度运行估算进入胶束和固定相的分配系数，以及随后在不同胶束浓度下的等度保留情况。然而，需要进行更多研究，以使等度数据和梯度数据之间达成更好的一致性。这些方程将有助于通过MLC高效开发实际分离方法。
