Madamba-Tan L S, Strasters J K, Khaledi M G
Department of Chemistry, North Carolina State University, Raleigh 27695-8204.
J Chromatogr A. 1994 Oct 21;683(2):335-45. doi: 10.1016/0021-9673(94)00451-X.
The application of organic modifier gradients in micellar liquid chromatography (MLC) is discussed. The equation derived by Snyder and co-workers describing gradient elution in hydro-organic reversed-phase LC was verified for organic solvent gradients in the presence of micelles. It is also demonstrated that the use of these gradients require little re-equilibration time due to the limited range of organic modifier concentration used in the gradient. This would result in shorter analysis time. Lastly, a practical application of the use of propanol and acetonitrile gradients in MLC is described.
讨论了有机改性剂梯度在胶束液相色谱（MLC）中的应用。验证了Snyder及其同事推导的描述水-有机反相液相色谱中梯度洗脱的方程在存在胶束的情况下对有机溶剂梯度的适用性。还证明，由于梯度中使用的有机改性剂浓度范围有限，使用这些梯度所需的重新平衡时间很少。这将导致分析时间缩短。最后，描述了在MLC中使用丙醇和乙腈梯度的实际应用。
