Grigioni W F, D'Errico A, Fiorentino M, Baccarini P, Onisto M, Caenazzo C, Stetler-Stevenson W G, Garbisa S, Mancini A M
Institute of Pathology, University of Bologna, Italy.
Diagn Mol Pathol. 1994 Sep;3(3):163-9. doi: 10.1097/00019606-199409000-00005.
Simultaneous presence of gelatinase A (MMP-2) and MMP-2 messenger RNA (mRNA) in 30 malignant tumors with various degrees of differentiation and biological behavior was evaluated by immunohistochemistry and in situ hybridization. The series consisted of 10 gastric carcinomas, 10 colorectal carcinomas, five squamous skin carcinomas, and five basal cell skin tumors. MMP-2 was detected in all cases. MMP-2 mRNA was expressed in the stromal cells in all cases and was more marked in the less-differentiated gastric and colonic carcinomas; it was also detected in the neoplastic cells of poorly differentiated tumors, particularly in those of the signet-ring cell type, both in the colon and stomach. The study confirmed that stromal cells have a specific role in tumor invasion and suggests a direct relationship between neoplastic epithelium and stromal cells in the most aggressive varieties.
通过免疫组织化学和原位杂交技术，对30例具有不同分化程度和生物学行为的恶性肿瘤中明胶酶A（基质金属蛋白酶-2，MMP-2）及其信使核糖核酸（mRNA）的同时存在情况进行了评估。该系列包括10例胃癌、10例结直肠癌、5例皮肤鳞状细胞癌和5例皮肤基底细胞瘤。所有病例均检测到MMP-2。所有病例的基质细胞中均表达MMP-2 mRNA，在低分化胃癌和结肠癌中更为明显；在低分化肿瘤的肿瘤细胞中也检测到MMP-2 mRNA，尤其是在结肠和胃的印戒细胞型肿瘤细胞中。该研究证实基质细胞在肿瘤侵袭中具有特定作用，并提示在侵袭性最强的肿瘤类型中，肿瘤上皮细胞与基质细胞之间存在直接关系。
