Tsukifuji R, Tagawa K, Hatamochi A, Shinkai H
Division of Dermatology, National Chiba Hospital, Japan.
Br J Cancer. 1999 Jun;80(7):1087-91. doi: 10.1038/sj.bjc.6690468.
Matrix metalloproteinase (MMP) plays an important role in extracellular matrix degradation associated with cancer invasion. An expression of MMP-1 (interstitial collagenase), MMP-2 (72-kDa type IV collagenase) and MMP-3 (stromelysin-1) was investigated in squamous cell carcinoma (SCC) and its precancerous condition, actinic keratosis (AK), using in situ hybridization techniques. MMP-1 mRNA was detected in tumour cells and/or in stromal cells in all cases of SCC, four of six AKs adjacent to SCC and four of 16 AKs. MMP-2 and MMP-3 mRNAs were detected in SCC but not in AK. The expression of MMP-3 correlated to that of MMP-1 (P = 0.03) localized at the tumour mass and stroma of the invasive area, while MMP-2 mRNA was detected widely throughout the stroma independent of MMP-1 expression. Our results indicated that the expression of MMP-1, -2 and -3 showed different localization patterns, suggesting a unique role of each MMP in tumour progression. Moreover, MMP-1 expression could be an early event in the development of SCC, and AK demonstrating MMP-1 mRNA, might be in a more advanced dysplastic state, progressing to SCC.
基质金属蛋白酶（MMP）在与癌症侵袭相关的细胞外基质降解中起重要作用。使用原位杂交技术，研究了基质金属蛋白酶-1（间质胶原酶）、基质金属蛋白酶-2（72 kDa IV型胶原酶）和基质金属蛋白酶-3（基质溶解素-1）在鳞状细胞癌（SCC）及其癌前病变光化性角化病（AK）中的表达情况。在所有SCC病例、6例与SCC相邻的AK中的4例以及16例AK中的4例中，在肿瘤细胞和/或基质细胞中检测到基质金属蛋白酶-1 mRNA。在SCC中检测到基质金属蛋白酶-2和基质金属蛋白酶-3 mRNA，但在AK中未检测到。基质金属蛋白酶-3的表达与位于侵袭区域肿瘤块和基质中的基质金属蛋白酶-1的表达相关（P = 0.03），而基质金属蛋白酶-2 mRNA在整个基质中广泛检测到，与基质金属蛋白酶-1的表达无关。我们的结果表明，基质金属蛋白酶-1、-2和-3的表达呈现不同的定位模式，提示每种基质金属蛋白酶在肿瘤进展中具有独特作用。此外，基质金属蛋白酶-1的表达可能是SCC发生过程中的早期事件，显示基质金属蛋白酶-1 mRNA的AK可能处于更高级的发育异常状态，进展为SCC。
