Linke R, Schwegler H, Boldyreva M
Anatomisches Institut, Universität Freiburg, Germany.
Brain Res. 1994 Aug 8;653(1-2):73-80. doi: 10.1016/0006-8993(94)90374-3.
The present experiments were directed to determine the proportions of the cholinergic and GABAergic septo-hippocampal projection neurons in NMRI mice. For the labeling of the septal neurons we used a double immunocytochemical method combined with retrograde transport of wheatgerm agglutinin apo-horseradish peroxidase-gold (WAHG) injected unilaterally into the hippocampus. Monoclonal antibodies against choline acetyltransferase (ChAT) and parvalbumin (PARV) were used as markers for cholinergic and GABAergic neurons in the medial septum/diagonal band complex (MS/DB). Both antibodies were visualized in the same section using the ABC detection system with diaminobenzidine and 4-chloro-1-naphtol as chromogens. Cholinergic and PARV-containing neurons are coexisting in the MS/DB. About 38% of all retrogradely labeled neurons were ChAT-positive whereas only 10% of all retrogradely labeled cells were immunostained for PARV. On the other hand, 40% of all ChAT-positive neurons and about 24% of all PARV-positive neurons were retrogradely labeled. No double immunolabeled neurons were detected. The proportion of GABAergic neurons may have been underestimated because immunostaining for PARV only labels a subpopulation of GABAergic neurons. The present results were compared with those of previous studies in rats. They may serve as a basis of further comparative studies in mice.
本实验旨在确定NMRI小鼠中胆碱能和γ-氨基丁酸能（GABAergic）隔区-海马投射神经元的比例。为标记隔区神经元，我们采用了一种双重免疫细胞化学方法，并结合将单侧注射到海马体中的麦胚凝集素脱辅基辣根过氧化物酶-金（WAHG）进行逆行运输。抗胆碱乙酰转移酶（ChAT）和小白蛋白（PARV）的单克隆抗体被用作内侧隔区/斜角带复合体（MS/DB）中胆碱能和GABA能神经元的标志物。使用以二氨基联苯胺和4-氯-1-萘酚作为显色剂的ABC检测系统，在同一切片中使两种抗体显色。胆碱能神经元和含PARV的神经元共存于MS/DB中。所有逆行标记神经元中约38%为ChAT阳性，而所有逆行标记细胞中只有10%对PARV进行了免疫染色。另一方面，所有ChAT阳性神经元中有40%以及所有PARV阳性神经元中有约24%被逆行标记。未检测到双重免疫标记的神经元。GABA能神经元的比例可能被低估了，因为对PARV的免疫染色仅标记了GABA能神经元的一个亚群。将本研究结果与先前对大鼠的研究结果进行了比较。它们可作为小鼠进一步比较研究的基础。
