Guo W, Soltesz V, Ding J W, Willén R, Liu X, Andersson R, Bengmark S
Department of Surgery, Lund University, Sweden.
Eur J Clin Invest. 1994 Aug;24(8):540-7. doi: 10.1111/j.1365-2362.1994.tb01104.x.
Biomaterials in the peritoneal cavity disrupt the physiology of the host and may cause bacterial translocation. The current study was performed to determine whether biomaterials exacerbate intra-abdominal infections. Adult male rats were divided into four groups: group 1, celiotomy+intraperitoneal (i.p.) saline; group 2, celiotomy+i.p. Escherichia coli (3 x 10(8) cfu); group 3, i.p. rubber+i.p. saline; and group 4, i.p. rubber+i.p. E. coli (3 x 10(8) cfu). Twelve h after the challenge, enteric bacterial translocation, bacterial population levels in the cecum and serum levels of IL-6 and TNF were measured. Bacterial translocation to mesenteric lymph nodes and the liver was observed in animals from groups 2 and 3, but significantly increased in group 4 with a concomitant elevation of serum levels of TNF and IL-6, as compared with group 1. Histological examination revealed a more pronounced inflammatory reaction in the peritoneum and distal ileum in group 4 than in groups 2 and 3. These results suggest that the presence of rubbers in the peritoneal cavity aggravates intra-abdominal sepsis.
腹腔内的生物材料会破坏宿主的生理机能，并可能导致细菌移位。本研究旨在确定生物材料是否会加剧腹腔内感染。成年雄性大鼠被分为四组：第1组，剖腹术+腹腔内（i.p.）注射生理盐水；第2组，剖腹术+i.p.注射大肠杆菌（3×10⁸ cfu）；第3组，i.p.注射橡胶+i.p.注射生理盐水；第4组，i.p.注射橡胶+i.p.注射大肠杆菌（3×10⁸ cfu）。在激发后12小时，测量肠道细菌移位、盲肠中的细菌数量水平以及IL-6和TNF的血清水平。在第2组和第3组的动物中观察到细菌向肠系膜淋巴结和肝脏的移位，但与第1组相比，第4组中细菌移位显著增加，同时血清TNF和IL-6水平升高。组织学检查显示，第4组腹膜和回肠末端的炎症反应比第2组和第3组更明显。这些结果表明，腹腔内橡胶的存在会加重腹腔内脓毒症。
