Jones C M, Poddar S, Goldstein R, Krupen K I, Kerman R H, Prince C, Shadduck R K
Department of Medicine, University of Texas Medical School at Houston.
Immunobiology. 1994 Jun;190(4-5):303-16. doi: 10.1016/S0171-2985(11)80604-2.
Monocyte cytotoxicity inducing factor (MCF) is a novel cytokine which was originally characterized by its ability to induce monocyte cytotoxicity against tumor targets. The present studies were undertaken to define the mechanisms of cytotoxicity. Since the cytotoxic state may be mediated, in part, by the secretion of monokines, MCF's effect on IL-1, TNF and CSF-1 were measured. MCF caused human peripheral blood monocytes to secrete large amounts of IL-1 but not TNF. In contrast monocytes produce CSF-1 in culture and this production was not enhanced by MCF. IL-1 neither appeared to directly lyse the target K562 nor was able to activate macrophages for cytotoxicity. However, IL-1 may be necessary for lysis of K562 by activated macrophages through its cytostatic effect on K562. MCF increased monocyte surface expression of DR-beta (when studied by fluorescent microfluorometry) and messenger RNA for DR-beta after 24 h in culture. These functional studies indicate that MCF is a cytokine with a distinct spectrum of biologic activities whose functions may be mediated by synthesis and secretion of IL-1.
单核细胞细胞毒性诱导因子（MCF）是一种新型细胞因子，最初因其诱导单核细胞对肿瘤靶标的细胞毒性的能力而被鉴定。目前的研究旨在确定细胞毒性的机制。由于细胞毒性状态可能部分由单核因子的分泌介导，因此检测了MCF对IL-1、TNF和CSF-1的影响。MCF可使人类外周血单核细胞分泌大量IL-1，但不分泌TNF。相比之下，单核细胞在培养中会产生CSF-1，且MCF不会增强这种产生。IL-1似乎既不能直接裂解靶标K562，也不能激活巨噬细胞产生细胞毒性。然而，IL-1可能是活化巨噬细胞裂解K562所必需的，因为它对K562有细胞生长抑制作用。培养24小时后，MCF增加了单核细胞表面DR-β的表达（通过荧光显微荧光测定法研究）以及DR-β的信使RNA。这些功能研究表明，MCF是一种具有独特生物活性谱的细胞因子，其功能可能由IL-1的合成和分泌介导。
