Adamska I, Kloppstech K
Institut für Botanik, Universität Hannover, Federal Republic of Germany.
J Biol Chem. 1994 Dec 2;269(48):30221-6.
Green pea plants respond to light stress by expression of a nuclear ELIP (early light-inducible protein) gene. Here we report that the accumulation of ELIP transcript in pea plants during light stress is enhanced by low temperature treatment. The enhanced level of ELIP transcript during combined light and cold stress was found to be due to an increased stability of ELIP messenger RNA under these conditions. This transcript is translatable in vitro. In vivo, however, the amount of accumulated protein in the thylakoids declines with the decrease in the temperature because the translational activity is strongly reduced already at 10 degrees C. Plants exposed to light stress at temperatures that do not allow accumulation of ELIP transcript respond by induction of ELIP mRNA and protein during recovery at low light intensity and ambient temperature. The amount of protein that accumulates as a result of this "memory effect" is, however, much lower than that which accumulates as a result of direct light stress. The memory of a perceived light stress persists in plants stored at low temperature for at least 3 h, and the stress response can be released after an increase in temperature. Prolonged cold treatment, however, has a negative effect on the translatability of the ELIP transcript that accumulates during recovery.
绿色豌豆植株通过核ELIP（早期光诱导蛋白）基因的表达来应对光胁迫。在此我们报告，低温处理可增强豌豆植株在光胁迫期间ELIP转录本的积累。发现在光和冷联合胁迫期间ELIP转录本水平的提高是由于在这些条件下ELIP信使RNA稳定性增加。该转录本在体外可翻译。然而在体内，类囊体中积累的蛋白量随温度降低而下降，因为翻译活性在10摄氏度时就已大幅降低。在不允许ELIP转录本积累的温度下遭受光胁迫的植株，在低光强度和环境温度下恢复期间会通过诱导ELIP mRNA和蛋白来做出反应。然而，这种“记忆效应”导致积累的蛋白量远低于直接光胁迫导致积累的蛋白量。感知到的光胁迫记忆在低温储存至少3小时的植株中持续存在，温度升高后胁迫反应可被释放。然而，长时间的冷处理对恢复期间积累的ELIP转录本的可翻译性有负面影响。
