Grundy M A, Moore K, Coakley W T
School of Pure and Applied Biology, University of Wales College Cardiff, UK.
J Immunol Methods. 1994 Dec 2;176(2):169-77. doi: 10.1016/0022-1759(94)90311-5.
A technique is described which increases the sensitivity of latex agglutination tests for soluble and particulate antigens. The levels of detection of tests for C-reactive protein and E. coli O157 respectively have been improved by x256 and x1024 compared with the standard test procedure of sample rotation on a test-card. This new method combines dilution of the test latex particles, a 2 min sample treatment in the ultrasonic standing wave field of a tubular piezo-electric transducer and subsequent examination by video-microscopy. Ultrasonic treatment is required to achieve increased localised concentrations of the latex particles in the standing wave field, and dilution of the latex is a critical requirement to allow agglutination to occur at low antigen concentrations.
本文描述了一种提高乳胶凝集试验对可溶性和颗粒性抗原敏感性的技术。与在测试卡上旋转样品的标准测试程序相比，C反应蛋白和大肠杆菌O157测试的检测水平分别提高了256倍和1024倍。这种新方法结合了测试乳胶颗粒的稀释、在管状压电换能器的超声驻波场中对样品进行2分钟处理以及随后通过视频显微镜进行检查。需要进行超声处理以在驻波场中实现乳胶颗粒局部浓度的增加，而乳胶的稀释是使凝集在低抗原浓度下发生的关键要求。
