Hechemy K, Stevens R W, Gaafar H A
Appl Microbiol. 1974 Aug;28(2):306-11. doi: 10.1128/am.28.2.306-311.1974.
A latex particle agglutination technique to detect ethylenediaminetetraacetate-solubilized extracts from Escherichia coli and whole E. coli cells is described. The sensitivity of the serological test was found to be 0.5 to 2.5 ng for the solubilized antigens and 1.5 x 10(6) to 5.7 x 10(6) cells per ml for the particulate antigens. The test was 100 to 1,000 times more sensitive than the standard bacterial agglutination test. Furthermore, it detected E. coli antigens during all phases of bacterial growth, whereas the bacterial test detected the antigens only after the mid-log phase. No significant cross-reactivity was observed between latex-anti-E. coli preparations and heterologous bacterial strains used in the experimental procedure. A buffer formula containing fatty acid-free bovine albumin prevented nonspecific aggregation of the latex particles.
本文描述了一种用于检测从大肠杆菌中提取的乙二胺四乙酸溶解物以及完整大肠杆菌细胞的乳胶颗粒凝集技术。血清学检测对溶解抗原的灵敏度为0.5至2.5纳克，对颗粒抗原的灵敏度为每毫升1.5×10⁶至5.7×10⁶个细胞。该检测比标准细菌凝集试验灵敏100至1000倍。此外，它能在细菌生长的所有阶段检测到大肠杆菌抗原，而细菌检测仅在对数中期后才能检测到抗原。在实验过程中，乳胶抗大肠杆菌制剂与异源细菌菌株之间未观察到明显的交叉反应。含有无脂肪酸牛血清白蛋白的缓冲液配方可防止乳胶颗粒的非特异性聚集。
