头孢曲松对人巨噬细胞吞噬和细胞内杀灭金黄色葡萄球菌的作用。

Effect of ceftriaxone on the phagocytosis and intracellular killing of Staphylococcus aureus by human macrophages.

作者信息

Tullio V, Cuffini A M, Cavallo R, Allocco A, Carlone N A

机构信息

Institute of Microbiology, University of Turin, Italy.

出版信息

J Chemother. 1994 Jun;6(3):177-83. doi: 10.1080/1120009x.1994.11741149.

DOI:10.1080/1120009x.1994.11741149

PMID:7983500

Abstract

The efficacy of an antimicrobial agent in the treatment of infections depends upon the interactions of bacteria, antibiotic and phagocytes. The influence of ceftriaxone on the phagocytic and bactericidal activity of human macrophages in vitro and ex vivo was investigated. At concentrations one-half the minimum inhibitory concentration (1/2 x MIC) the antibiotic caused in vitro a significant enhancement of phagocytosis and a reduction in the survival of intracellular Staphylococcus aureus. The distinction between any effect of ceftriaxone on the staphylococci and the macrophages was made by exposure of each of them to the antibiotic before they were incubated together. The results suggest that ceftriaxone may have a direct positive action on macrophages, possibly by interfering with the cellular membrane functions and hence enhancing engulfment of bacteria. The ex vivo data seem to corroborate this hypothesis.

摘要

抗菌剂在治疗感染中的疗效取决于细菌、抗生素和吞噬细胞之间的相互作用。研究了头孢曲松对人巨噬细胞体外和体内吞噬及杀菌活性的影响。在浓度为最低抑菌浓度的一半（1/2×MIC）时，该抗生素在体外显著增强了吞噬作用，并降低了细胞内金黄色葡萄球菌的存活率。通过在将它们一起孵育之前分别将头孢曲松与葡萄球菌和巨噬细胞接触，区分了头孢曲松对葡萄球菌和巨噬细胞的任何作用。结果表明，头孢曲松可能对巨噬细胞有直接的积极作用，可能是通过干扰细胞膜功能从而增强对细菌的吞噬作用。体内数据似乎证实了这一假设。