Khoury M A, Goldberg V M, Stevenson S
Department of Orthopaedics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
J Orthop Res. 1994 Nov;12(6):751-7. doi: 10.1002/jor.1100120602.
The expression of HLA and ABH antigens in fresh, frozen, and twice-frozen normal human meniscal tissue was evaluated with use of immunohistochemistry. A generalized expression of Class-I and Class-II HLA antigens was found in the endothelial and synovial cells of all three forms of tissue. Fibrochondrocytes were Class-I positive and Class-II negative. ABH antigens were expressed by endothelial cells in relationship to the blood group of the patient. Freezing preserved most of the HLA and ABH molecular structure. Although the normal meniscus consists of a relatively few chondrocytes embedded in an extracellular matrix, it also contains Class-II and ABH-positive endothelial cells and Class-II-positive synovial cells. These antigens are present at the moment of transplantation and could evoke an immune response in the host that would modulate the results of meniscal allografting.
采用免疫组织化学方法评估新鲜、冷冻及二次冷冻的正常人半月板组织中HLA和ABH抗原的表达情况。在所有三种组织形式的内皮细胞和滑膜细胞中均发现了I类和II类HLA抗原的普遍表达。纤维软骨细胞I类阳性、II类阴性。ABH抗原由内皮细胞根据患者血型表达。冷冻保存了大部分HLA和ABH分子结构。尽管正常半月板由嵌入细胞外基质中的相对较少的软骨细胞组成，但它也含有II类和ABH阳性的内皮细胞以及II类阳性的滑膜细胞。这些抗原在移植时就已存在，可能会在宿主中引发免疫反应，从而调节半月板同种异体移植的结果。
