Togni G, Sanglard D, Monod M
Laboratoire de Mycologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
J Med Vet Mycol. 1994;32(4):257-65. doi: 10.1080/02681219480000331.
The relationship between the ability to secrete a specific acid proteinase (ACP) by Candida tropicalis in the presence of bovine serum albumin as a nitrogen source and virulence for mice was studied using two stable proteinase-positive and proteinase-negative strains (DSY68 and DSY65), which were constructed from the wild-type pathogenic yeast C. tropicalis ATCC 750. The inactivation of the gene encoding the secreted acid proteinase was produced by targeted gene disruption. Mortality rate was slightly lower in groups of mice infected with the proteinase-negative mutant. All other parameters analysed were similar for two strains of yeast. Our results therefore conclude that the ACP secreted by C. tropicalis did not contribute significantly to fungal virulence in systemic infections.
利用从野生型致病性热带假丝酵母ATCC 750构建的两个稳定的蛋白酶阳性和蛋白酶阴性菌株（DSY68和DSY65），研究了热带假丝酵母在以牛血清白蛋白作为氮源的情况下分泌特定酸性蛋白酶（ACP）的能力与对小鼠的毒力之间的关系。通过靶向基因破坏使编码分泌性酸性蛋白酶的基因失活。感染蛋白酶阴性突变体的小鼠组死亡率略低。分析的所有其他参数在两种酵母菌株中相似。因此，我们的结果得出结论，热带假丝酵母分泌的ACP在全身感染中对真菌毒力没有显著贡献。
