Caraballo I, Fernandez-Arevalo M, Holgado M A, Vela M T, Rabasco A M
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Seville, Spain.
J Pharm Sci. 1994 Aug;83(8):1147-9. doi: 10.1002/jps.2600830815.
A rapid, sensitive, and accurate reversed-phase HPLC method has been developed for the analysis and quantification of pharmaceutical formulations containing tyrothricin (1), an antibiotic used in antiseptic buccal compressed tablets for local application. The assay has been carried out under isocratic conditions, using a stationary phase of alumina particles coated with polybutadiene and an alkaline mobile phase (pH = 8.2). No HPLC method was reported for the analysis of 1. So, this new technique is an alternative to the slow and tedious microbiological methods. On the other hand, it allows the simultaneous quantification of 1, benzocaine (2), and menthol (3), an aromatic compound not currently analyzed by liquid chromatography.
已开发出一种快速、灵敏且准确的反相高效液相色谱法，用于分析和定量含有短杆菌肽（1）的药物制剂，短杆菌肽是一种用于局部应用的防腐口腔压制片的抗生素。该测定在等度条件下进行，使用涂覆有聚丁二烯的氧化铝颗粒固定相和碱性流动相（pH = 8.2）。尚未有用于分析1的高效液相色谱法报道。因此，这项新技术是缓慢且繁琐的微生物学方法的一种替代方法。另一方面，它能够同时定量1、苯佐卡因（2）和薄荷醇（3），薄荷醇是一种目前未通过液相色谱分析的芳香化合物。
