De Vita S, Ferraccioli G, De Re V, Dolcetti R, Carbone A, Bartoli E, Boiocchi M
Division of Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano (PN), Italy.
J Rheumatol. 1994 Aug;21(8):1497-501.
To investigate the usefulness of the polymerase chain reaction (PCR) to detect B cell clonal expansions in tissues where lymphoproliferative disease was suspected in the course of Sjögren's syndrome (SS).
Tissue samples from 7 patients with definite (5 cases) or probable (2 cases) SS were subjected to routine histopathologic studies, immunophenotyping, and genotypic analysis, including Southern blotting and PCR amplification of immunoglobulin heavy chain (IgH) variable-diversity-joining (VDJ) region gene rearrangements.
Malignant lymphoma was detected in 3 cases, and myoepithelial sialadenitis in the remaining 4. B cell clonal expansion was detected in 7/7 cases by PCR, 5/7 by Southern blotting, and in no case of myoepithelial sialadenitis by kappa/lambda light chain immunophenotyping.
PCR may represent a quick and powerful tool to detect B cell clonalities in SS. Such information may provide new insights into the pathogenesis of the disease and may improve the clinical approach to the patients.
研究聚合酶链反应（PCR）在检测干燥综合征（SS）病程中疑似淋巴增殖性疾病的组织中B细胞克隆性扩增的实用性。
对7例确诊（5例）或可能（2例）SS患者的组织样本进行常规组织病理学研究、免疫表型分析和基因分型分析，包括Southern印迹法和免疫球蛋白重链（IgH）可变区-多样性区-连接区（VDJ）基因重排的PCR扩增。
3例检测出恶性淋巴瘤，其余4例为肌上皮性涎腺炎。通过PCR在7/7例中检测到B细胞克隆性扩增，通过Southern印迹法在5/7例中检测到，而在肌上皮性涎腺炎病例中，κ/λ轻链免疫表型分析未检测到B细胞克隆性扩增。
PCR可能是检测SS中B细胞克隆性的一种快速且强大的工具。此类信息可能为该疾病的发病机制提供新的见解，并可能改善对患者的临床治疗方法。
