1型人类免疫缺陷病毒Nef蛋白诱导CD4在早期内体中积累。
Human immunodeficiency virus type 1 Nef induces accumulation of CD4 in early endosomes.
作者信息
Schwartz O, Dautry-Varsat A, Goud B, Maréchal V, Subtil A, Heard J M, Danos O
机构信息
Laboratoire Rétrovirus et Transfert Génétique (URA CNRS 1157), Paris, France.
出版信息
J Virol. 1995 Jan;69(1):528-33. doi: 10.1128/JVI.69.1.528-533.1995.
Abstract
We have studied the fate of CD4 in CEM T cells expressing a human immunodeficiency virus type 1 HIV-1 Nef protein. Nef triggered a rapid endocytosis and a degradation of CD4, while most of the p56lck was upheld at the cell membrane. In the presence of Nef, CD4 accumulated in acidic intracellular vesicles that were not stained by antibodies against rab6, a marker of the Golgi apparatus complex. Detection of transferrin in CD4-containing vesicles showed that CD4 was trapped in early endosomes, without significant accumulation of CD4 in late endocytic compartments. Internalization pathways taken by CD4 in Nef+ cells may therefore be different from those observed after treatment with phorbol esters.
摘要
我们研究了在表达1型人类免疫缺陷病毒（HIV-1）Nef蛋白的CEM T细胞中CD4的命运。Nef引发了CD4的快速内吞作用和降解，而大多数p56lck保留在细胞膜上。在Nef存在的情况下，CD4积聚在酸性细胞内囊泡中，这些囊泡未被针对高尔基体复合物标记物rab6的抗体染色。在含有CD4的囊泡中检测转铁蛋白表明，CD4被困在早期内体中，在晚期内吞区室中没有明显的CD4积累。因此，Nef+细胞中CD4所采用的内化途径可能与佛波酯处理后观察到的途径不同。
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