Ohagi S, Yoshida H, Nanjo K
First Department of Medicine, Wakayama University of Medical Science.
Nihon Rinsho. 1994 Oct;52(10):2544-9.
Prohormone Convertase 2 (PC2) is a specific endoprotease responsible for the processing of proinsulin to insulin. PC2 is expressed in pancreatic islets, pituitary and brain but is very low or absent in most other tissues, such as liver, spleen and kidney. To evaluate the regulated expression of the human PC2 gene we have analyzed its structure and characterized its promoter. The gene spans > 130 kilobase pairs and consists of 12 exons. Comparison with the structure of the gene encoding human furin, revealed a high degree of conservation of exon-intron junctions. The hPC2 gene was localized to chromosome 20, band p11.2. The promoter region of the PC2 gene is very G + C rich and contains six potential Sp1 binding sites but no TATA or CAAT box. Analysis of the level of chrolamphenicol acetyltransferase activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for the PC2 promoter activity.
激素原转化酶2（PC2）是一种特异性内切蛋白酶，负责将胰岛素原加工成胰岛素。PC2在胰岛、垂体和大脑中表达，但在大多数其他组织如肝脏、脾脏和肾脏中表达非常低或不表达。为了评估人PC2基因的调控表达，我们分析了其结构并对其启动子进行了表征。该基因跨度超过130千碱基对，由12个外显子组成。与编码人弗林蛋白酶的基因结构比较，发现外显子-内含子连接具有高度保守性。hPC2基因定位于20号染色体p11.2带。PC2基因的启动子区域富含G + C，包含六个潜在的Sp1结合位点，但没有TATA或CAAT框。用几个缺失突变体分析氯霉素乙酰转移酶活性水平，确定从翻译起始位点-1100至-539的区域对PC2启动子活性至关重要。
