Ohagi S, Tatsuta H, Sanke T, Furuta H, Furuta M, Hanabusa T, Nanjo K
The First Department of Medicine, Wakayama University of Medical Science, Japan.
Endocr J. 1998 Aug;45(4):569-73. doi: 10.1507/endocrj.45.569.
Prohormone convertase 2 (PC2) is a member of a family of mammalian subtilisin-like endoproteases that are involved in the processing of prohormones, neuropeptides and many other precursor derived proteins. The expression of PC2 is restricted to neuroendocrine tissues such as pancreatic islets, the pituitary and the brain. To understand the regulation of the PC2 gene, we cloned and characterized the promoter region of the mouse PC2 gene. The transcriptional start site of the mouse PC2 gene is identical to that of the human. There is 79% identity in the sequences of the promoter regions between the mouse and human PC2 genes. The mouse PC2 gene, like the human, does not have a TATA-like motif in the region just upstream of the start of the transcription. Studies with promoter-reporter gene, chloramphenicol acetyltransferase (CAT), constructs showed that the region from -400 to -170 bp was necessary for high level expression of the mouse PC2 gene in the betaTC-3 insulinoma cells.
激素原转化酶2（PC2）是哺乳动物枯草杆菌蛋白酶样内切蛋白酶家族的成员，该家族参与激素原、神经肽和许多其他前体衍生蛋白的加工过程。PC2的表达仅限于神经内分泌组织，如胰岛、垂体和大脑。为了解PC2基因的调控机制，我们克隆并鉴定了小鼠PC2基因的启动子区域。小鼠PC2基因的转录起始位点与人的相同。小鼠和人PC2基因启动子区域的序列有79%的同一性。与人类一样，小鼠PC2基因在转录起始点上游区域没有类似TATA的基序。用启动子-报告基因氯霉素乙酰转移酶（CAT）构建体进行的研究表明，-400至-170 bp区域对于小鼠PC2基因在βTC-3胰岛素瘤细胞中的高水平表达是必需的。
